棘孢木霉酸性GH12木葡聚糖酶水解产生益生元寡糖并

来源:Front Nutr | 编译:DNA Lab Space

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木葡聚糖作为植物半纤维素的主要成分,其酶解产生的寡糖具有潜在益生元活性。当前研究从棘孢木霉中筛选并异源表达了一种酸性GH12木葡聚糖酶,系统表征了其酶学性质,证明该酶能够高效水解木葡聚糖并释放低聚合度寡糖,且这些寡糖可显著促进乳杆菌和双歧杆菌等益生菌的体外生长。该工作为酸性条件下利用植物多糖制备功能性食品配料提供了新酶源,也拓展了木葡聚糖寡糖在肠道微生态调控中的应用前景。

人类肠道内居住着数量庞大、种类繁多的微生物群落,它们与宿主营养代谢、免疫防御和疾病发生密切相关。通过膳食摄入益生元来选择性

Multiple sequence alignment of TaXEG12 (GenBank accession No. XAM95724 ) with other GH12 xyloglucanases, including Asper
▲ Multiple sequence alignment of TaXEG12 (GenBank accession No. XAM95724 ) with other GH12 xyloglucanases, including Asper
Optimized expression of TaXEG12 in K. phaffii . (A) Recombinant strains TaXEG12-wt and TaXEG12-opt (schematic). (B) Enzy
▲ Optimized expression of TaXEG12 in K. phaffii . (A) Recombinant strains TaXEG12-wt and TaXEG12-opt (schematic). (B) Enzy
Effects of pH and temperature on TaXEG12 activity with tamarind xyloglucan as substrate. (A) Optimal pH. (B) pH stabilit
▲ Effects of pH and temperature on TaXEG12 activity with tamarind xyloglucan as substrate. (A) Optimal pH. (B) pH stabilit

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